Investigation of Stoichiometry of T4 Bacteriophage Helicase Loader
WebMay 25, 2012 · From bacteriophage T4 to eukaryotes, these factors include a helicase to unwind the DNA ahead of the replication fork, a single-stranded binding protein (SSB) to bind to the ssDNA on the lagging strand, and a helicase loader that associates with the fork, helicase, and SSB.
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Investigation of Stoichiometry of T4 Bacteriophage Helicase Loader
Websubunit stoichiometry, and assembly pathway of the primosome complex of the bacteriophage T4 DNA replication system. Analyti-cal ultracentrifugation and fluorescence anisotropy methods show that the functional T4 primosome consists of six gp41 helicase sub-units that assemble into a hexagon, driven by the binding of six
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Investigation of Stoichiometry of T4 Bacteriophage Helicase Loader
WebJan 29, 2005 · The T4 helicase-loading protein (gp59) has been proposed to coordinate leading- and lagging-strand DNA synthesis by blocking leading-strand synthesis during the primosome assembly.
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Mutational analysis of the T4 gp59 helicase loader reveals its …
WebThe T4 bacteriophage helicase loader (gp59) is one of the main eight proteins that play an active role in the replisome. gp59 is a small protein (26 kDa) that exists as a monomer in solution and in the crystal. It binds preferentially to forked DNA and interacts directly with the T4 helicase (gp41), single-stranded DNA-binding protein (gp32), and polymerase (gp43).
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Bacteriophage T4 gene 41 helicase and gene 59 helicase-loading …
WebMay 1, 2005 · Interaction between the T4 helicase loading protein (gp59) and the DNA polymerase (gp43): unlocking of the gp59-gp43-DNA complex to initiate assembly of a fully functional replisome. Sign in | Create an account. https://orcid.org. Europe PMC
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Interaction between the T4 Helicase-Loading Protein (gp59) and …
WebJul 8, 2012 · Investigation of Stoichiometry of T4 Bacteriophage Helicase Loader Protein (gp59)* American Society for Biochemistry and Molecular Biology. Abstract. The T4 bacteriophage helicase loader (gp59) is one of the main eight proteins that play an active role in the replisome. gp59 is a small protein (26 kDa) that exists as a monomer in …
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Investigation of Stoichiometry of T4 Bacteriophage Helicase Loader
WebThe T4 bacteriophage helicase loader (gp59) is one of the main eight proteins that play an active role in the replisome. gp59 is a small protein (26 kDa) that exists as a monomer in solution and in the crystal. It binds preferentially to forked DNA and interacts directly with the T4 helicase (gp41), single-stranded DNA-binding protein (gp32), and polymerase (gp43). …
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Insights into Okazaki Fragment Synthesis by the T4 Replisome
WebThe bacteriophage T4 59 protein plays an essential role in recombination and replication by mediating the assembly of the gene 41 helicase (gp41) onto DNA, and the architecture and stoichiometry of the gp41-gp59 complex were investigated. The bacteriophage T4 59 protein (gp59) plays an essential role in recombination and replication by mediating the …
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Investigation of Stoichiometry of T4 Bacteriophage Helicase Loader
WebPossessing the fundamental set of protein constituents common to eukaryotic counterparts, the T4 bacteriophage DNA replisome has long served as a model for understanding the DNA replication process. The current working model of the T4 system faithfully portrays the mode of action of replisomes in general.
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Investigation of stoichiometry of T4 bacteriophage helicase loader
WebMentioning: 15 - The T4 bacteriophage helicase loader (gp59) is one of the main eight proteins that play an active role in the replisome. gp59 is a small protein (26 kDa) that exists as a monomer in solution and in the crystal. It binds preferentially to forked DNA and interacts directly with the T4 helicase (gp41), singlestranded DNA-binding protein (gp32), …
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Assembly and subunit stoichiometry of the functional …
WebJul 17, 2001 · The genes for each of the T4 replication proteins have been cloned, and the functions of the proteins have been characterized by in vitro reactions on model templates (Fig. 1) (reviewed in ref. 5).T4 DNA polymerase (gene 43), which catalyzes DNA synthesis on both leading and lagging strands, is attached to a clamp protein (gene 45) that is …
Get a quoteAssembly of the bacteriophage T4 helicase: architecture and
WebDNA helicase loader protein that participates in viral DNA replication, recombination, and repair (PubMed:10871615). At the fork, required for loading of the replicative helicase onto DNA protected by the ssDNA-binding protein (PubMed:11459967, PubMed:7806533, PubMed:10871615, PubMed:22427673). Coordinates simultaneous synthesis of leading- …
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Models for the Binary Complex of Bacteriophage T4 Gp59 Helicase …
WebThe T4 replisome has provided a unique opportunity to investigate the intricacies of DNA replication. We present a comprehensive review of this system focusing on the following: its 8-protein composition, their individual and synergistic activities, and assembly in vitro and in vivo into a replisome capable of coordinated leading/lagging strand DNA synthesis. We …
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Investigation of Stoichiometry of T4 Bacteriophage Helicase Loader
WebIn this study, single-molecule photobleaching experiments were employed to elucidate the stoichiometry of gp59 on a forked DNA and to investigate its interaction with other proteins forming the primosome complex. The T4 bacteriophage helicase loader (gp59) is one of the main eight proteins that play an active role in the replisome. gp59 is a small protein …
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Investigation of stoichiometry of T4 bacteriophage helicase loader
WebApr 5, 1970 · Investigation of stoichiometry of T4 bacteriophage helicase loader protein (gp59). J Biol Chem. 2009; 284(43):29283-9 (ISSN: 1083-351X) Arumugam SR; Lee TH; Benkovic SJ
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Assembly of the bacteriophage T4 helicase: architecture and
WebDNA helicase loader protein that participates in viral DNA replication, recombination, and repair (PubMed:10871615). At the fork, required for loading of the replicative helicase onto DNA protected by the ssDNA-binding protein (PubMed:11459967, PubMed:7806533, PubMed:10871615, PubMed:22427673). Coordinates simultaneous synthesis of leading- …
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Understanding DNA replication by the bacteriophage T4 replisome
WebAug 21, 2012 · Physical biochemical techniques are used to establish the structure, subunit stoichiometry, and assembly pathway of the primosome complex of the bacteriophage T4 DNA replication system. Analytical ultracentrifugation and fluorescence anisotropy methods show that the functional T4 primosome consists …
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Investigation of stoichiometry of T4 bacteriophage helicase loader
WebThe T4 bacteriophage helicase loader (gp59) is one of the main eight proteins that play an active role in the replisome. gp59 is a small protein (26 kDa) that exists as a monomer in solution and in the crystal.
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Interaction between the T4 Helicase-Loading Protein (gp59) and …
WebAug 5, 2003 · A ring breaker mechanism has been demonstrated for bacteriophage T7 gp4A helicase–primase [5]. The loader of the archaeal 'mini-chromosome maintenance' (MCM) helicase may also be a ring breaker, as it is a stable double hexamer [6] and is active for DNA unwinding over a large range of protein concentrations [7].
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Investigation of Stoichiometry of T4 Bacteriophage Helicase Loader
WebThe T4 bacteriophage helicase loader (gp59) is one of the main eight proteins that play an active role in the replisome. gp59 is a small protein (26 kDa) that exists as a monomer in solution and in the crystal. It binds preferentially to forked DNA and interacts directly with the T4 helicase (gp41), single-stranded DNA-binding protein (gp32), and polymerase (gp43). …
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